All products Antibodies Alkaline Phosphatase [SA40-00]

Alkaline Phosphatase [SA40-00]

Catalog Number：OM626575

  • Fig1: Western blot analysis of ALP on different cell lysates using anti-ALP antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MG-63

  • Fig2: ICC staining ALP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Fig9: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Fig10: Flow cytometric analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Product Profile

Product Name Alkaline Phosphatase [SA40-00]
Antibody Type Primary Antibodies
Product description 
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Placental ALP is highly polymorphic, there are at least three common alleles. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Immunogen 
recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications

WBICCIHCIPFC
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
Alkaline phosphatase antibody Alkaline phosphatase placental antibody Alkaline phosphatase placental type antibody Alkaline phosphatase Regan isozyme antibody ALP antibody Alp1 antibody ALPP antibody FLJ61142 antibody Germ-cell alkaline phosphatase antibody nagao isozyme antibody OTTHUMP00000164354 antibody PALP antibody Placental alkaline phosphatase 1 antibody placental heat-stable alkaline phosphatase antibody placental type antibody PLAP antibody PLAP-1 antibody PLAP1 antibody PPB1_HUMAN antibody
Molecular Weight(MW) 57 kDa
Cellular Localization Cell membrane

Database Links

SwissProt ID P05186
P09242
P08289

Application

  • Application

    Fig1: Western blot analysis of ALP on different cell lysates using anti-ALP antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MG-63

  • Application

    Fig2: ICC staining ALP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Application

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-ALP antibody. Counter stained with hematoxylin.

  • Application

    Fig10: Flow cytometric analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Positive Control SW480, MG-63, Hela, HepG2, mouse kidney tissue, human liver tissue, mouse small intestine tissue, human liver cancer tissue, human kidney tissue.
Application Notes
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626575
  • Price: $386.00
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  • Lead time: 4~6 Week

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